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親水作用液色譜(Hydrophilic Interaction LiquidChromatography,HILIC) 是一種直接分離極性和親水性化合物的色譜技術(shù),為了便于理解,我們可以把它看作為是一種反相色譜溶劑做為流動(dòng)相的正相色譜。因此HILIC是:● 親水性的固定相● 以水、有機溶劑和緩沖液作為流動(dòng)相洗脫劑 常見(jiàn)的HILIC方法采用50-90%有機溶劑
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相關(guān)文章Merck ZIC-HILIC 親水作用色譜柱
● 直接分離極性和親水性化合物
超長(cháng)的耐受性使得樣品分離的成本很低
● ZIC-pHILIC,聚合物基質(zhì),PH2-10
為什么使用HILIC?
盡管反相液相色譜(RPLC)是至今應用zui廣的色譜分離技術(shù),它能與各種常規的檢測方法結合,解決多種分析應用問(wèn)題,但對某些分析物,特別是極性和親水性化合物卻無(wú)法或很少保留。長(cháng)期以來(lái),人們采用正相液相色譜(NPLC),并使用不利環(huán)保的非水溶性流動(dòng)相如己脘或環(huán)己脘來(lái)分析這些物質(zhì)。
但是在這種實(shí)驗條件下,很多極性和親水化合物往往很難溶解,從而限制了NPLC的應用范圍。從現在起有了另一種新的解決辦法,即選用親水作用液相色譜-HILIC。
什么是親水作用色譜(HILIC):
親水作用液色譜(Hydrophilic Interaction LiquidChromatography,HILIC) 是一種直接分離極性和親水性化合物的色譜技術(shù),為了便于理解,我們可以把它看作為是一種反相色譜溶劑做為流動(dòng)相的正相色譜。
因此HILIC是:
● 親水性的固定相
● 以水、有機溶劑和緩沖液作為流動(dòng)相洗脫劑
常見(jiàn)的HILIC方法采用50-90%有機溶劑和一定比例的水,并且含有低濃度的緩沖鹽。如乙腈/水/醋酸銨體系
在HILIC方法中,洗脫順序和反相色譜正好相反,即極性越大,保留時(shí)間越長(cháng)。
訂貨信息: | |
貨號 | 描述 固定相 規格 |
1.50434.0001 | ZIC?-HILIC (5μm) 14 x 1 mm PEEK保護柱(5件) fitting guard column |
1.50435.0001 | ZIC?-HILIC (5μm) 20 x 2.1 mm PEEK保護柱 (1件) fitting guard column |
1.50436.0001 | ZIC?-HILIC (5μm) 20 x 2.1 mm PEEK保護柱套件 (3件) fitting guard column |
1.50439.0001 | ZIC?-HILIC (3.5μm, 100?) 20 x 2.1 mm PEEK HPLC column |
1.50440.0001 | ZIC?-HILIC (3.5μm, 100?) 50 x 2.1 mm PEEK HPLC column |
1.50441.0001 | ZIC?-HILIC (3.5μm, 100?) 100 x 2.1 mm PEEK HPLC column |
1.50442.0001 | ZIC?-HILIC (3.5μm, 100?) 150 x 2.1 mm PEEK HPLC column |
1.50444.0001 | ZIC?-HILIC (3.5μm, 100?) 150 x 4.6 mm PEEK HPLC column |
1.50443.0001 | ZIC?-HILIC (3.5μm, 100?) 250 x 2.1 mm PEEK HPLC column |
1.50445.0001 | ZIC?-HILIC (3.5μm, 200?) 50 x 2.1 mm PEEK HPLC column |
1.50446.0001 | ZIC?-HILIC (3.5μm, 200?) 50 x 4.6 mm PEEK HPLC column |
1.50447.0001 | ZIC?-HILIC (3.5μm, 200?) 100 x 2.1 mm PEEK HPLC column |
1.50448.0001 | ZIC?-HILIC (3.5μm, 200?) 150 x 2.1 mm PEEK HPLC column |
1.50449.0001 | ZIC?-HILIC (3.5μm, 200?) 150 x 4.6 mm PEEK HPLC column |
1.50450.0001 | ZIC?-HILIC (5μm, 200?) 50 x 2.1 mm PEEK HPLC column |
1.50451.0001 | ZIC?-HILIC (5μm, 200?) 50 x 4.6 mm PEEK HPLC column |
1.50452.0001 | ZIC?-HILIC (5μm, 200?) 100 x 2.1 mm PEEK HPLC column |
1.50453.0001 | ZIC?-HILIC (5μm, 200?) 100 x 4.6 mm PEEK HPLC column |
1.50454.0001 | ZIC?-HILIC (5μm, 200?) 150 x 2.1 mm PEEK HPLC column |
1.50455.0001 | ZIC?-HILIC (5μm, 200?) 150 x 4.6 mm PEEK HPLC column |
1.50456.0001 | ZIC?HILIC (5μm, 200?) 150 x 7.5 mm PEEK HPLC column |
1.50457.0001 | ZIC?-HILIC (5μm, 200?) 250 x 2.1 mm PEEK HPLC column |
1.50458.0001 | ZIC?-HILIC (5μm, 200?) 250 x 4.6 mm PEEK HPLC column |
1.50437.0001 | ZIC?-pHILIC polymeric (5μm) 20 x 2.1 mm PEEK Guard (1 pc) PEEK保護柱(1件) |
1.50438.0001 | ZIC?-pHILIC polymeric (5μm) 20 x 2.1 mm PEEK Guard Kit PEEK保護柱套件(3件) |
1.50462.0001 | ZIC?-pHILIC polymeric (5μm) 100 x 2.1 mm PEEK metal-free HPLC column |
1.50460.0001 | ZIC?-pHILIC polymeric (5μm) 150 x 2.1 mm PEEK metal-free HPLC column |
1.50464.0001 | ZIC?-pHILIC polymeric (5μm) 100 x 4.6 mm PEEK metal-free HPLC column |
1.50463.0001 | ZIC?-pHILIC polymeric (5μm) 50 x 4.6 mm PEEK metal-free HPLC column |
1.50459.0001 | ZIC?-pHILIC polymeric (5μm) 50 x 2.1 mm PEEK metal-free HPLC column |
1.50461.0001 | ZIC?-pHILIC polymeric (5μm) 150 x 4.6 mm PEEK metal-free HPLC column |