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英文名稱(chēng):Waters ACQUITY UPLC Column型號規格:ACQUITY UPLC BEH HILIC Column, 130Å, 1.7 µm, 2.1 mm X 150 mm貨號:186003462品牌:沃特世/Waters
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相關(guān)文章品牌 | Waters/沃特世 |
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ACQUITY UPLC 色譜柱和 VanGuard 保護柱是迄今為止技術(shù)的液相色譜柱。產(chǎn)品經(jīng)過(guò)專(zhuān)門(mén)設計和檢驗,確保可用于高達 15000 psi (1000 bar)的壓力,具有的高柱效和耐用性。有超過(guò) 60 種不同規格和不同鍵合相的產(chǎn)品供選。分離科學(xué)家可以充分利用小顆粒填料的優(yōu)勢,在獲得更高分離度的同時(shí)得到更快的分離。
- 超快速分離
- 超高分離度
- 超高靈敏度
- 行業(yè)的設計與制造技術(shù)
- 容易從HPLC條件轉移到UPLC條件
- 使用壽命長(cháng),重現性好
ACQUIT Y UPLC 顆粒技術(shù)
制造 UPLC 顆粒比合成小顆粒需要更多的技術(shù),絕大多數 HPLC 顆粒都不能保持在超高效液相色譜操作壓力(例如:15000 psi/1000 bar)條件下的機械穩定性和結構完整性。為什么顆粒的耐壓性質(zhì)如此重要呢?為了*實(shí)現亞 2 微米顆粒的高柱效,需要在更高的線(xiàn)速度下(亦即更高體積流速)下進(jìn)行日常分離,而亞 2 微米小顆粒在更高的線(xiàn)速度條件下會(huì )產(chǎn)生更高的操作壓力。沃特世創(chuàng )造了兩種高效耐壓的 UPLC顆粒:1.7 μm 亞乙基橋雜化顆粒(BEH)和 1.8 μm 高強度硅膠顆粒(HSS)。
*個(gè) ACQUITY UPLC 顆粒是 1.7 μm 亞乙基橋雜化顆粒(BEH),這是沃特世第二代雜化顆粒,它是 UPLC 技術(shù)的一個(gè)關(guān)鍵推動(dòng)力,共有 5 種不同的鍵合相:C18, C8, Shield RP18,Phenyl 和 HILIC。由于它是雜化顆粒,因此具有更寬的 pH 使用范圍(pH 1-12),能夠幫助分離科學(xué)家快速有效地開(kāi)發(fā)方法。同時(shí),BEH 顆粒技術(shù)也用于 XBridge 系列的 HPLC色譜柱,有不同粒徑(2.5、3.5、5 和 10 μm)供選,因此分離方法能夠在 HPLC 和 UPLC 技術(shù)平臺實(shí)現無(wú)縫轉換。
第二個(gè) ACQUITY UPLC 顆粒是 1.8μm 的高強度硅膠顆粒(HSS),共有三種不同的鍵合相:HSS C18 、HSS C18 SB (Selectivity for Bases)和 T3。HSS C18 是全封端的超高效通用型的C18 柱:為堿性化合物提供良好的峰形;同 ACQUITY UPLC BEH C18 柱相比有更強的保留能力;在酸性流動(dòng)相條件下具有超長(cháng)的使用壽命。HSS C18 SB(Selectivity for Bases)是未經(jīng)封端處理的 C18 柱,設計用于低 pH 條件下方法開(kāi)發(fā),它提供互補的分離選擇性,特別針對堿性化合物,具有與現代高覆蓋率 C18 柱不同的分離選擇性。HSS T3 色譜柱是與 100 % 水性流動(dòng)相兼容的 C18 柱,專(zhuān)門(mén)用來(lái)保留和分離極性水溶性好的有機小分子,很像 Atlantis T3 HPLC 柱。
UPLC顆粒技術(shù)參數 | ||||||||
亞乙基橋雜化顆粒(BEH) | 高強度硅膠顆粒(HSS) | |||||||
粒徑 | 1.7 μm | 1.8 μm | ||||||
孔徑/孔體積 | 130? / 0.7 mL/g | 100? / 0.7 mL/g | ||||||
比表面積 | 185 m2/g | 230 m2/g | ||||||
zui高耐壓 | 15000 psi (~1000 bar) | 15000 psi (~1000 bar) | ||||||
UPLC鍵合相技術(shù)參數 | ||||||||
BEH顆粒 | HSS顆粒 | |||||||
鍵合相 | C18 | C8 | Shield RP 18 | 苯基 | HILIC | C18 | C18 SB | T3 |
碳含量* | 18% | 13% | 17% | 15% | - | 15% | 8% | 11% |
封端 | 專(zhuān)有 | 專(zhuān)有 | TMS | 專(zhuān)有 | - | 專(zhuān)有 | 無(wú) | 專(zhuān)有 |
技術(shù) | ||||||||
pH使用范圍 | 1-12 | 1-12 | 2-11 | 1-12 | 1-8 | 1-8 | 2-8 | 2-8 |
*大約值 | ||||||||
ACQUITY UPLC 訂貨信息 | ||||||||
鍵合相 | 規格 | 粒徑 | 部件號 | 部件號 | ||||
1根/包 | 3根/包 | |||||||
BEH C18 | 1.0×50mm | 1.7μm | 186002344 | 176000861 | ||||
1.0×100mm | 1.7μm | 186002346 | 176000862 | |||||
1.0×150mm | 1.7μm | 186002347 | 176001044 | |||||
2.1×30mm | 1.7μm | 186002349 | 176001304 | |||||
2.1×50mm | 1.7μm | 186002350 | 176000863 | |||||
2.1×100mm | 1.7μm | 186002352 | 176000864 | |||||
2.1×150mm | 1.7μm | 186002353 | 176001048 | |||||
BEH Shield RP18 | 1.0×50mm | 1.7μm | 186002851 | 176000874 | ||||
1.0×100mm | 1.7μm | 186002852 | 176000875 | |||||
1.0×150mm | 1.7μm | 186003373 | 176001045 | |||||
2.1×30mm | 1.7μm | 186003909 | 176001309 | |||||
2.1×50mm | 1.7μm | 186002853 | 176000876 | |||||
2.1×100mm | 1.7μm | 186002854 | 176000877 | |||||
2.1×150mm | 1.7μm | 186003376 | 176001049 | |||||
BEH C8 | 1.0×50mm | 1.7μm | 186002875 | 176000882 | ||||
1.0×100mm | 1.7μm | 186002876 | 176000883 | |||||
1.0×150mm | 1.7μm | 186003374 | 176001046 | |||||
2.1×30mm | 1.7μm | 186003910 | 176001310 | |||||
2.1×50mm | 1.7μm | 186002877 | 176000884 | |||||
2.1×100mm | 1.7μm | 186002878 | 176000885 | |||||
2.1×150mm | 1.7μm | 186003377 | 176001050 | |||||
BEH 苯基 | 1.0×50mm | 1.7μm | 186002882 | 176000905 | ||||
1.0×100mm | 1.7μm | 186002883 | 176000906 | |||||
1.0×150mm | 1.7μm | 186003375 | 176001047 | |||||
2.1×30mm | 1.7μm | 186003911 | 176001311 | |||||
2.1×50mm | 1.7μm | 186002884 | 176000907 | |||||
2.1×100mm | 1.7μm | 186002885 | 176000908 | |||||
2.1×150mm | 1.7μm | 186003378 | 176001051 | |||||
BEH HILIC | 1.0×50mm | 1.7μm | 186003457 | 176001089 | ||||
1.0×100mm | 1.7μm | 186003458 | 176001090 | |||||
1.0×150mm | 1.7μm | 186003459 | 176001091 | |||||
2.1×50mm | 1.7μm | 186003460 | 176001092 | |||||
2.1×100mm | 1.7μm | 186003461 | 176001093 | |||||
2.1×150mm | 1.7μm | 186003462 | 176001094 | |||||
HSS C18 | 1.0×50mm | 1.8μm | 186003529 | 176001121 | ||||
1.0×100mm | 1.8μm | 186003530 | 176001122 | |||||
1.0×150mm | 1.8μm | 186003531 | 176001123 | |||||
2.1×30mm | 1.8μm | 186003987 | 176001398 | |||||
2.1×50mm | 1.8μm | 186003532 | 176001124 | |||||
2.1×100mm | 1.8μm | 186003533 | 176001125 | |||||
2.1×150mm | 1.8μm | 186003534 | 176001126 | |||||
HSS C18 SB | 1.0×50mm | 1.8μm | 186004114 | 176001556 | ||||
1.0×100mm | 1.8μm | 186004115 | 176001557 | |||||
1.0×150mm | 1.8μm | 186004116 | 176001558 | |||||
2.1×30mm | 1.8μm | 186004117 | 176001559 | |||||
2.1×50mm | 1.8μm | 186004118 | 176001560 | |||||
2.1×100mm | 1.8μm | 186004119 | 176001561 | |||||
2.1×150mm | 1.8μm | 186004120 | 176001562 | |||||
HSS T3 | 1.0×50mm | 1.8μm | 186003535 | 176001127 | ||||
1.0×100mm | 1.8μm | 186003536 | 176001129 | |||||
1.0×150mm | 1.8μm | 186003537 | 176001375 | |||||
2.1×30mm | 1.8μm | 186003944 | 176001130 | |||||
2.1×50mm | 1.8μm | 186003538 | 176001131 | |||||
2.1×100mm | 1.8μm | 186003539 | 176001132 | |||||
2.1×150mm | 1.8μm | 186003540 | 176001133 | |||||
ACQUITY UPLC 色譜柱組合包(4 根/包) | ||||||||
描述 | 規格 | 部件號 | ||||||
高至低 pH 、zui寬的分離選擇性 UPLC 柱組合包 | 2.1 x 50 mm | 176001042 | ||||||
BEH C18, BEH C8, BEH Shield RP18, BEH Phenyl | 2.1 x 100 mm | 176001043 | ||||||
UPLC 方法開(kāi)發(fā)柱組合包 | 2.1 x 50 mm | 176001603 | ||||||
BEH C18, BEH Shield RP18, BEH Phenyl, HSS T3 | 2.1 x 100 mm | 176001604 | ||||||
USP L1 UPLC 柱組合包 | 2.1 x 50 mm | 176001605 | ||||||
BEH C18, BEH Shield RP18, HSS C18, HSS T3 | 2.1 x 100 mm | 176001606 | ||||||
質(zhì)譜分析用 UPLC 柱組合包 | 2.1 x 50 mm | 176001607 | ||||||
BEH C18, HSS C18, HSS C18 SB, HSS T3 | 2.1 x 100 mm | 176001608 | ||||||
低 pH、zui寬的分離選擇性 UPLC 柱組合包 | 2.1 x 50 mm | 176001609 | ||||||
BEH Shield RP18, BEH Phenyl, HSS C18, HSS C18 SB | 2.1 x 100 mm | 176001610 | ||||||
ACQUI TY UPLC 柱方法驗證組合包* | ||||||||
鍵合相 | 規格 | 粒徑 | 部件號 | |||||
BEH C18 | 2.1 x 50 mm | 1.7 μm | 186004044 | |||||
2.1 x 100 mm | 1.7 μm | 186004045 | ||||||
BEH C8 | 2.1 x 50 mm | 1.7 μm | 186004046 | |||||
2.1 x 100 mm | 1.7 μm | 186004047 | ||||||
BEH Shield RP18 | 2.1 x 50 mm | 1.7 μm | 186004048 | |||||
2.1 x 100 mm | 1.7 μm | 186004049 | ||||||
BEH 苯基 | 2.1 x 50 mm | 1.7 μm | 186004050 | |||||
2.1 x 100 mm | 1.7 μm | 186004052 | ||||||
BEH HILIC | 2.1 x 50 mm | 1.7 μm | 186004053 | |||||
2.1 x 100 mm | 1.7 μm | 186004054 | ||||||
HSS C18 | 2.1 x 50 mm | 1.8 μm | 186004057 | |||||
2.1 x 100 mm | 1.8 μm | 186004058 | ||||||
HSS C18 SB | 2.1 x 50 mm | 1.8 μm | 186004137 | |||||
2.1 x 100 mm | 1.8 μm | 186004138 | ||||||
HSS T3 | 2.1 x 50 mm | 1.8 μm | 186004055 | |||||
2.1 x 100 mm | 1.8 μm | 186004056 | ||||||
* 含有 3 根不同批次填料的柱子 | ||||||||
VanGuard 一體式保護柱(用于保護 ACQUITY UPLC 色譜柱,3個(gè)/盒) | ||||||||
鍵合相 | 規格 | 粒徑 | 部件號 | |||||
BEH C18 | 2.1 x 5 mm | 1.7 μm | 186003975 | |||||
BEH Shield RP18 | 2.1 x 5 mm | 1.7 μm | 186003977 | |||||
BEH C8 | 2.1 x 5 mm | 1.7 μm | 186003978 | |||||
BEH 苯基 | 2.1 x 5 mm | 1.7 μm | 186003979 | |||||
BEH HILIC | 2.1 x 5 mm | 1.7 μm | 186003980 | |||||
HSS C18 | 2.1 x 5 mm | 1.8 μm | 186003981 | |||||
HSS C18 SB | 2.1 x 5 mm | 1.8 μm | 186004136 | |||||
HSS T3 | 2.1 x 5 mm | 1.8 μm | 186003976 |